Efficient High-Density Cell Banking

Using Single-Use Fluoropolymer Bags

The need for rapid development and scale-up of vaccines and therapeutics has never been greater. But conventional seed train processes used to amplify cells from cell cultures are inefficient and subject to risk. Improving efficiency requires increasing the volume of media in cell cultures, the cell density, or both, and reducing the number of process steps.

Research on high-density cell banking (HDCB) has demon­strated its ability to increase the density in cell cultures from 10 million cells per mL up to 100 million cells per mL. With HDCB you get:

  • high cell concentrations
  • larger volumes
  • storage at -150oC
  • direct inoculation in larger bioreactors
  • considerable upstream processing time, space, and cost savings

This innovative process eliminates many of the labor intensive and risky approaches currently used by most bioprocessing labs. By eliminating manual transfer steps, high-density cell banking minimizes contamination risk. Instead of storing frozen cell cultures in individual vials, high-density cell banking relies on larger vessels.

When replacing cell cryopreservation containers with another storage method, the cells must be protected throughout the freezing, storage, thawing, and transfer processes. Single-use 2D storage bag assemblies made from fluoropolymers offer a solution that increases both cell culture volume and cell density per mL while preserving cell viability.